FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH4_MTS0mS4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH4_MTS0mS4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2342913
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG26050.060.3228156
TATAGGC18200.058.2646337
CTATAGG16100.057.8227546
CTTATAG13800.057.8227545
CGGATAG6800.057.6437385
TATAGGG23650.057.5641637
GTATAGG23500.056.741416
CGTATAG13050.056.5867775
TCTACGG4750.055.9955757
TATAGGT32350.055.7143067
TATAGTG33300.055.280927
GGTATAG20900.055.092525
TTATAGT29800.054.8458336
CGTATAA11300.054.8198475
GCGATAG7400.054.388765
TTTATAG28150.054.2064675
GATAGGT12850.053.9257057
GTTATAG28850.053.8617175
TATAGTC19950.053.5045477
ATAGGGC8400.053.329128
Produced by FastQC (version 0.11.5)