FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH49_MTS60mS3_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH49_MTS60mS3_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3239645
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG44000.054.963916
CTTATAG20500.054.2907075
GCGATAG9750.053.8441935
GTATAGG40700.053.057046
TATAGGG47650.052.1492047
GGTATAG37000.051.741365
GTTATAG44450.051.5729265
TTATAGT49350.051.203816
CGTATAA14050.051.065765
TTATAAG29550.051.0472156
ATAGGGC13450.050.7408648
CGATAGG8850.050.619636
CTATAGG18700.050.532926
TTTATAG44750.050.3668825
TATAAGG31300.049.982217
CGGATAG10750.049.812145
TATAGTG53500.049.7832377
GTATAAG29500.049.7100566
TATAGGT45250.049.500967
GGGATAG25250.049.483355
Produced by FastQC (version 0.11.5)