FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH48_MTS60mS2_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH48_MTS60mS2_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4092148
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG48000.057.894526
CTTATAG23150.056.9965485
CGTATAA20250.056.517245
GCGATAG12400.055.885835
CGATAGG11250.055.376526
GTATAGG42450.055.1577576
TATAGGG46800.054.9640127
CGTATAG21450.054.6607635
CGATAGT13800.054.274136
CGGATAG11350.053.9635355
CTATAGG25450.053.22086
TTATAGT54000.052.3691836
GGTATAG37750.052.197495
TATAGGC27400.051.9853327
TATAGGT55200.051.5452237
GTTATAG50050.051.257585
ATAGGGC14250.050.8384678
TATAGTG57300.050.450147
GCGTATA23050.050.2591634
TTTATAG51800.050.201565
Produced by FastQC (version 0.11.5)