FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH47_MTS60mS1_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH47_MTS60mS1_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4316896
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG49900.059.6865046
GTATAGG45750.058.9042636
TATAGGG49650.057.6602027
CTTATAG25400.057.5956545
CGTATAG21850.057.5029535
CTATAGG29350.057.4759376
CGGATAG14400.057.1153075
CGTATAA21050.056.3630835
GGTATAG42600.054.3870545
GCGATAG12550.054.3798565
TTATAGT56850.054.1750536
GATAGGT25700.054.062927
AGTATAG33250.053.786885
GTTATAG52000.053.776265
TATAGTG66650.053.717727
TATAGGT63150.053.646837
TCTACGG8700.053.5025527
TATAGGC30650.053.4388967
GCGTATA26850.053.051454
TATAGGA42450.053.012157
Produced by FastQC (version 0.11.5)