FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH43_MTS45mS3_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH43_MTS45mS3_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6156138
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG38150.057.0599175
TTATAGG74900.056.3976176
CTATAGG38750.055.9957776
CGGATAG19050.055.6650925
CGTATAG32800.055.3769235
GGTATAG59350.054.8400085
GTATAGG66850.054.341456
CGTATAA32450.054.03295
TATAGGC47300.053.4217837
TATAGGG76050.053.3827747
TATAGGT79700.053.265357
CGATAGG17350.053.252476
ATAGGCG20350.053.1413848
TTATAGT77100.052.836486
TATAGTG85000.052.085157
ATAGGGC26500.051.6380358
TTATAAG58150.051.5782246
TATAAGG58600.051.541327
GCGATAG18900.051.47765
TTTATAG73250.051.3135385
Produced by FastQC (version 0.11.5)