FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH42_MTS45mS2_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH42_MTS45mS2_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5960096
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG69700.056.238836
CTTATAG36100.055.7457355
CGTATAG30600.055.3573045
CGTATAA28200.054.6077885
CGGATAG16400.054.4185945
GTATAGG58600.054.170236
TATAGGT76850.053.8751227
TATAGGG69750.053.7895557
GGTATAG58400.053.5766645
CTATAGG37950.052.9360056
CGATAGT19700.051.8761376
TTATAGT82150.051.7204366
GCGATAG19900.051.7065165
TATAGTG90800.051.0713587
TTATAAG54850.051.0463146
CGATAGG18550.050.9413956
GTTATAG76950.049.6666685
TATAGGC43200.049.580977
GATAGGT38200.049.3824657
TATAAGG57150.049.2977457
Produced by FastQC (version 0.11.5)