FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH41_MTS45mS1_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH41_MTS45mS1_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3492422
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG44150.058.8182686
GTATAGG36500.058.297476
CGGATAG10800.056.7091675
CTTATAG20650.056.2674265
TATAGGG42000.055.9129267
TATAGGT44250.055.758987
GTTATAG42900.055.3925675
GGTATAG34150.055.340375
GCGATAG11750.055.1026955
CGTATAA17800.054.6592675
TTATAGT44100.054.3614466
CGTATAG19900.054.3430985
TATAGTG47550.053.582127
GATAGGT21750.053.2608037
TTATAAG32000.053.1526956
TATAGGC27000.053.0149737
CGTTATA24100.052.7143174
CTATAGG23600.052.3481946
GTATAAG29550.052.230016
TATAAGG34650.052.1177257
Produced by FastQC (version 0.11.5)