FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH3_MTS0mS3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH3_MTS0mS3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5806761
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG17400.058.330935
TTATAGG74100.057.0084426
CGTATAG30350.055.582555
GTATAGG60250.055.242546
GGTATAG60250.055.126365
CTATAGG38750.054.823556
TATAGGG73500.054.5192157
CTTATAG35300.054.332045
CGTATAA28100.052.3110125
ATAGGGC26100.052.159918
TTATAAG54900.052.0197146
TTATAGT75150.051.6013346
GATAGGT36300.051.483277
TATAGGT77100.051.1111267
TTTATAG72150.050.7394185
GTTATAA55350.050.711555
CGATAGG17300.050.575956
TATAGTG80150.050.432437
CGTTATA39200.050.3550644
TATAGGC46050.050.3106357
Produced by FastQC (version 0.11.5)