FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH37_MTS30mS3_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH37_MTS30mS3_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4593396
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG54300.058.1379176
GTATAGG46200.057.6494876
CTATAGG28650.057.04866
TATAGGG53400.056.887537
CGGATAG15050.056.7421885
CGTATAG24650.055.799255
CGTATAA21400.054.46075
CTTATAG27850.054.4146045
GGTATAG43950.053.9116445
TATAGTG69300.053.5822267
TCTACGG9500.053.0490387
GCGATAG16300.053.0349435
TATAGGT63400.052.9380537
TATAGGC33550.052.887627
CGATAGG14200.052.744626
TTATAGT65100.052.471276
CGATAGT17150.052.0389866
GTTATAG59350.052.011655
AGTATAG34500.051.94025
ATAGGGC17900.051.812138
Produced by FastQC (version 0.11.5)