FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH36_MTS30mS2_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH36_MTS30mS2_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4888843
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG58850.057.6251686
CGGATAG13250.057.5805135
TATAGGG58850.056.19857
GTATAGG53650.056.1653336
CTATAGG28400.055.453516
GCGATAG15450.054.5913055
CGATAGT15600.054.515076
GGTATAG48950.054.122535
CTTATAG29450.053.9517445
TATAGGT69000.053.61247
CGTATAA23150.053.3652575
ATAGGGC17350.053.051258
CGATAGG13450.052.8212476
CGTATAG27150.052.7215775
GTTATAG64650.052.671935
TATAGTG83100.052.517597
TTATAGT68600.052.2919276
ATAGGTG42900.051.0688868
TCTACGG9950.050.6499187
GATAGGT29650.050.5194327
Produced by FastQC (version 0.11.5)