FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH35_MTS30mS1_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH35_MTS30mS1_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8419667
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG110800.059.6045236
CTTATAG51850.059.5346035
CGGATAG26000.059.2282565
GTATAGG93150.059.1007356
CGTATAA40200.057.808495
CTATAGG62150.057.2136466
GGTATAG85700.057.132935
TTATAGT105950.056.4531486
CGATAGG26500.056.39356
TATAGGG108550.056.0672237
TATAGGT130800.056.0016487
CGTATAG48000.055.924715
GCGATAG28300.055.156675
GTTATAG103550.054.821435
TCTACGG21600.054.7648967
TATAGGC65900.054.488057
AGTATAG67750.053.9829525
TTTATAG105650.053.7985
TTATAAG77650.053.094666
TATAGTG123600.052.6097877
Produced by FastQC (version 0.11.5)