FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH31_MTS20mS3_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH31_MTS20mS3_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5234062
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG62700.059.72676
CTTATAG32950.058.5259445
CTATAGG32700.056.5117076
GTATAGG51350.056.3660166
CGTATAG25950.055.836125
TATAGGG61150.055.5739067
GCGATAG14700.055.236095
CGGATAG15000.055.064675
CGATAGT16450.054.6788676
TATAGGT71450.054.5181437
CCTATAC12650.054.503955
TATAGTG77750.054.4219447
CGTATAA26350.054.191585
TATAGGC37500.054.130857
TTATAGT76900.054.0227476
GGTATAG49550.052.6217045
TTTATAG67500.051.124075
GTTATAG71700.051.058125
AGTATAG40750.050.2435725
ATAGGGC19150.050.0760358
Produced by FastQC (version 0.11.5)