FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH30_MTS20mS2_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH30_MTS20mS2_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3513659
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG42700.061.5534746
CTTATAG19050.060.2586445
GTATAGG41000.059.4962276
CTATAGG23400.058.030516
TTATAGT47300.057.7870336
GGTATAG38650.057.4090355
CGTATAG20450.057.3313265
CGTATAA17600.056.871395
TATAGGT50450.056.6762857
TATAGGG40550.056.6178677
CGGATAG10700.056.58525
GTTATAG43450.056.383085
CGATAGG8550.055.6689876
CGATAGT10850.055.157796
TATAGGC27800.055.0144927
TTTATAG43750.054.7965245
TATAGTC27800.054.510937
TATAGCG22100.054.476187
TATAGTG52400.054.366787
ATTATAG38350.054.1165165
Produced by FastQC (version 0.11.5)