FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH2_MTS0mS2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH2_MTS0mS2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4179190
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG21150.058.41235
CGGATAG12750.057.9178285
GTATAGG44650.056.514386
TTATAGG48800.056.0831156
CTTATAG24550.055.7397775
CGTATAA20300.055.5136575
TATAGGG52450.054.248167
GGTATAG40650.054.2400325
CGATAGG12250.053.9971586
CTATAGG29050.053.8525856
TTATAAG37750.053.7720076
GCGATAG13350.053.479695
TCTACGG10900.052.978237
TATAGTG52950.052.744477
TATAGGT56000.052.0591357
TATAGGC34250.052.011247
TTATAGT50250.051.7485246
CGATAGT12550.051.590916
CATATAG35200.051.3035055
GTTATAG50900.051.2245865
Produced by FastQC (version 0.11.5)