FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH29_MTS20mS1_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH29_MTS20mS1_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3404624
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG42600.060.5496446
CTTATAG22950.060.2376635
CGGATAG9550.057.5373735
TATAGGG40050.056.9743927
CTATAGG20950.056.96716
GCGATAG11250.056.6203585
GTATAGG37750.055.9054456
TTATAGT47250.055.4055826
CGTATAG19100.055.3384935
ATAGGGC11650.054.9743088
CGATAGT10200.054.900156
CGTATAA18100.054.7219545
CGATAGG9850.054.719016
GTTATAG43950.054.7080965
GGTATAG33400.054.070085
TATAGTG50400.053.4681057
TCTACGG8650.053.4062927
AGTATAG25950.053.1389055
TATAGGT48100.052.96897
CCTATAC7950.052.8284535
Produced by FastQC (version 0.11.5)