FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH25_MTS15mS3_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH25_MTS15mS3_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6272563
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG75600.056.386796
CTTATAG36750.055.4265065
TATAGGG75800.054.851097
CGGATAG18750.054.504645
CGTATAA29350.054.376175
GTATAGG68050.054.1566936
CTATAGG41500.054.0582246
CGTATAG33150.053.316275
GGTATAG61550.053.0524525
TATAGGT77100.052.564467
GCGATAG21800.052.33755
TTATAAG56200.052.1243936
CGTTATA43950.051.6021734
ATAGGGC25000.051.096068
TTATAGT74400.050.9456836
GTTATAG74150.050.6454475
CGATAGG17350.050.43046
TATAGGC48150.050.2976657
ATTATAG70000.050.148135
CGATAGT21500.049.9748846
Produced by FastQC (version 0.11.5)