FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH24_MTS15mS2_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH24_MTS15mS2_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4670229
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG59800.060.047686
CGGATAG13150.059.3511545
GTATAGG47400.059.0692836
CTTATAG28700.058.046375
CTATAGG27150.057.36416
TATAGGG53400.057.216737
GGTATAG46900.056.2663275
TTATAGT66100.055.224646
TATAGGT65400.055.013017
CGATAGG11600.054.911526
TATAGGC31650.054.847657
CGTATAA21450.054.659745
CGTATAG25550.054.5182885
ATAGGGC17700.054.1785438
GTTATAG62150.053.4410675
GCGATAG13050.053.369445
AGTATAG34750.053.278375
GCGATAA10550.052.4148865
TATAGTG69400.052.3969657
CCTATAC8900.052.30125
Produced by FastQC (version 0.11.5)