FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH23_MTS15mS1_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH23_MTS15mS1_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3335858
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG19250.063.633245
TTATAGG32400.062.7592666
CGTATAG17850.062.7420235
GTATAGG33900.062.2535826
GTTATAG36900.060.701635
CTATAGG21050.060.3533336
TTATAGT35650.059.688526
CGATAGT9000.059.497086
GCGATAG9600.059.4241685
TATAGGC22900.058.68627
CCTATAC7700.058.633495
TATAGGG34450.058.5158467
CGTATAA16900.058.39955
TATAGGT38450.058.071717
TATAGTG44750.057.2477347
AGTATAG21400.057.2401855
TTATAAG26700.057.1507576
GCTATAG24350.056.0547455
ATTATAG29000.055.876575
GCGTATA23350.055.4576644
Produced by FastQC (version 0.11.5)