FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH1_MTS0mS1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH1_MTS0mS1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5374643
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG69550.057.013756
CTTATAG31450.055.8637245
TATAGGG67400.054.989387
CGGATAG17200.054.7356955
GCGATAG16800.054.580665
CGTATAG27650.054.4277155
GTATAGG56200.054.116576
CTATAGG35000.053.397396
GGTATAG54250.052.4490475
CGTATAA25850.051.989725
TTATAAG48450.051.8653686
ATAGGGC22750.051.8431368
TTATAGT70650.051.2713746
TATAGTG78150.051.052697
GTTATAG67000.050.7736325
CGATAGG14550.050.7535326
GATAGGT33750.050.3970687
TATAGGT70450.050.1745077
CGATAGA14300.049.6828846
CGTTATA37000.049.659734
Produced by FastQC (version 0.11.5)