FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH19_MTS10mS3_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH19_MTS10mS3_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5422567
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG62400.056.2001156
CTTATAG31050.055.4571535
CGGATAG15300.054.6714445
GTATAGG59950.054.4686436
GGTATAG50000.054.2482575
CGTATAG29250.054.0837335
TATAGGG69050.053.878027
CTATAGG36400.053.7482726
CGTATAA27850.052.6553965
GCGATAG17100.052.3959775
TATAGGC42350.052.1456457
TTATAGT68950.051.5211836
TATAGGT68100.051.4433257
TCTACGG13550.051.140857
TTATAAG49000.051.0697866
GTTATAG66150.050.5274735
CGATAGG15400.050.452926
GCGTATA33050.050.3009534
GGTTATA56100.049.9092674
CGTTATA35450.049.8573534
Produced by FastQC (version 0.11.5)