FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH18_MTS10mS2_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH18_MTS10mS2_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6833565
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG77150.054.1644676
GGTATAG63250.053.7285045
CTATAGG39900.053.418356
CGGATAG20050.053.4137575
GTATAGG68700.053.286976
CTTATAG37650.053.264245
TATAGGG80350.053.138657
CGTATAA30550.052.4687085
CGTATAG36650.052.4257245
GCGATAG20500.052.0705385
CGATAGG16650.051.709096
TTATAGT86800.050.64266
TATAGTG92000.050.4419947
TATAGGT80700.050.306147
CCTATAC13550.049.5915875
TTATAAG61550.049.5831956
TATAGGC47700.049.5247087
CGATAGT21650.049.466326
TCTACGG17450.049.3373957
GCGTATA38400.048.8516924
Produced by FastQC (version 0.11.5)