FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH17_MTS10mS1_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH17_MTS10mS1_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6381465
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG77700.056.1680036
CGTATAG33850.055.3144575
CTTATAG39250.054.6593365
TATAGGG76800.053.7274367
GTATAGG62600.053.447466
CGGATAG19400.053.3990485
CGTATAA30600.052.6114125
GGTATAG61050.052.454055
CTATAGG37800.051.848936
CGATAGG19850.051.8358656
GTTATAG78850.050.9990235
TATAGTG88950.050.9527177
TATAGGT80800.050.9377257
GCGATAG20250.050.8119475
TTATAGT81650.050.4074446
ATAGGGC24150.050.2874158
GCGATAA18950.049.6805735
CGTTATA45650.049.4495664
TTATAAG54850.049.386466
GGTTATA68650.049.1450774
Produced by FastQC (version 0.11.5)