FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH13_MTS5mS3_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH13_MTS5mS3_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3914782
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG48700.061.299556
CGGATAG10700.061.1638875
CTTATAG23950.060.4967465
CTATAGG26650.059.095246
CGTATAG23450.058.5033075
CGTATAA19000.058.0221945
TATAGGG46500.057.7256137
GTATAGG41800.057.5198366
GGTATAG38150.056.5097545
TTATAGT51050.056.421076
CGATAGG13000.056.265246
TATAGGT58400.056.2103277
ATTATAG41100.055.4340175
CCTATAC8850.055.36335
GTTATAG47550.055.201135
GCGATAG12700.055.1141975
TATAGGC29200.055.0118147
AGTATAG30900.054.9313775
TTTATAG47000.054.357845
CGATAGT12950.054.320476
Produced by FastQC (version 0.11.5)