FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH12_MTS5mS2_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH12_MTS5mS2_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6074477
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG18450.058.9955755
TTATAGG72200.057.9276776
CGTATAG30450.057.6994745
GTATAGG61600.057.3278856
CTTATAG36150.057.024625
CTATAGG38950.056.6093946
TATAGGG64350.055.6366657
CGTATAA28000.055.4983945
GCGATAG19250.054.9074975
CGATAGG16600.054.8176886
TATAGTG88500.054.0974547
TATAGGC45400.054.0376137
TTATAGT83200.053.9287226
GGTATAG55500.053.917355
CGATAGT19750.053.5174336
TATAGGT85100.053.133327
GTTATAG74200.052.545645
ATAGGCG18350.051.8760388
ATAGGGC22750.051.688258
AGTATAG49000.051.284225
Produced by FastQC (version 0.11.5)