FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH121_MTS120mS3_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH121_MTS120mS3_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3438399
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG38800.058.4527246
CGTATAG17850.058.430495
GTATAGG36300.057.6575286
CTTATAG20350.057.6158375
GGTATAG32000.056.2178965
CTATAGG24700.055.8291176
CGGATAG9900.055.1506775
TATAGGG39550.054.600157
CGTATAA17400.054.5106665
TATAGGC29100.054.4829147
CCTATAC7200.054.4436235
GCGATAG10350.054.4436155
TATAGGT44500.054.1107527
AGTATAG25900.053.9181025
ATAGGGC14300.053.8437738
TTATAAG29850.053.466536
GTTATAA29850.053.466535
CGATAGG10700.053.3169486
ATAGGCG11500.053.258518
TTATAGT41200.053.1788066
Produced by FastQC (version 0.11.5)