FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH120_MTS120mS2_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH120_MTS120mS2_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3895569
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG39100.057.9121066
CTTATAG23700.057.7391325
CGTATAA18950.057.621855
GGTATAG36850.057.3642465
TTATAGG44600.057.048136
CGGATAG11350.057.0450175
GCGATAG12200.056.7998545
CGATAGG11050.056.6934136
CTATAGG26000.056.265846
CGTATAG19300.055.6702165
TATAGGG46800.055.6383867
CCTATAC8000.053.8092545
TATAGGT49750.053.6758427
TATAGTG51500.053.2110637
AGTATAG31950.053.1266865
GTTATAG45550.053.0154655
ATAGGCG14000.052.497518
TTATAGT49200.052.4968346
TATAAGG36400.052.016767
GTTATAA34650.051.8150565
Produced by FastQC (version 0.11.5)