FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH11_MTS5mS1_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH11_MTS5mS1_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5864412
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG18900.058.145145
CTTATAG35250.057.089255
TTATAGG71050.056.0562176
CGTATAA29300.055.543215
GTATAGG60300.055.3122336
CTATAGG38800.054.5719266
GGTATAG54750.054.271175
CGTATAG32800.053.6710055
TATAGGG71500.053.598177
TATAGGT72800.052.641067
TTATAGT73350.051.9605066
TATAGTG79750.051.783727
GCGTATA35900.051.7661254
TTATAAG53200.051.642076
GTTATAG72750.051.5231135
CGATAGG16400.051.4302676
GCGATAG17700.051.2120445
GATAGGT34600.051.080747
TATAGGC43950.050.963947
CGCTATA27850.050.895044
Produced by FastQC (version 0.11.5)