FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH119_MTS120mS1_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH119_MTS120mS1_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306939
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG14150.058.1225665
TTATAGG23450.056.7118876
CGGATAG7150.056.2892045
CGTATAG10850.056.1245465
TATAGGG21700.054.99567
GTATAGG17950.054.981686
CTATAGG13000.054.918696
CCTATAC5800.053.7026065
TTATAGT28100.052.558086
TTATAAG22500.052.4180346
GCGATAG5550.051.707585
TATAGGT26500.051.5053187
ATAGGGC7750.051.479768
GCGTATA11850.051.0929684
CGATAGT8100.050.983586
TATAGCG13150.050.8324437
CGTTATA15150.050.359014
TTTATAG27450.050.2327845
GGTATAG17600.050.1096345
CGATAGG5800.050.0822076
Produced by FastQC (version 0.11.5)