FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH115_MTS90mS3_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH115_MTS90mS3_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3710505
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG45500.058.148326
CTTATAG21550.057.975925
GGTATAG38900.055.5988245
CGGATAG12600.055.5502745
GTATAGG40550.055.2351886
CGTATAA17400.055.10975
CGATAGG8400.054.5781446
TATAGGG41000.053.946097
GCGATAG10850.053.5432935
TATAGGT51800.052.9679347
CGTATAG20150.052.451595
TTATAGT46950.052.327296
ATAGGCG12450.052.2841878
GTTATAG49050.052.227455
ATAGGGC14200.052.0020948
TATAGTG53350.051.7570277
CTATAGG23500.051.6759386
GCGTATA22400.051.5575944
CGTTATA26000.051.5527884
TTATAAG33950.050.8199126
Produced by FastQC (version 0.11.5)