FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH114_MTS90mS2_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH114_MTS90mS2_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767298
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG18000.058.721396
CGTATAA9750.058.5119975
CGGATAG4700.058.0842865
TATAGGG15900.057.6705067
GGTATAG13650.056.922275
GTATAGG14600.056.814276
TCTACGG3150.056.664267
CTTATAG10900.056.5129625
CTATAGG9400.056.222616
GCGATAA5300.055.4709135
TATAGGT20000.054.24777
TTATAGT21750.054.229126
AGTATAG12400.053.6282735
CGTTATA12300.053.495184
CGTATAG9200.053.2601135
CCTATAC4800.053.2284135
CGCTATA9700.053.040494
CGATAGT5650.052.654126
CTATAAC7450.052.6167076
TATAGTC14450.052.5583237
Produced by FastQC (version 0.11.5)