FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH113_MTS90mS1_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH113_MTS90mS1_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2995790
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG19950.060.6961825
CGTATAA14150.058.36925
GTATAGG31200.058.327986
TTATAGG37300.058.0777786
CGTATAG15350.057.9099925
CTATAGG22600.057.605336
CGGATAG9200.057.0599825
GGTATAG25950.056.9119225
TATAGGC24950.056.5288247
TATAGGG40800.056.098737
CTATAAC11000.055.6767046
CATATAG25350.055.635925
TATAGTC25500.055.584067
TTATAAG26850.055.004266
TTATAGT38300.054.551136
CCTATAC5550.054.2292565
GCGATAG8800.054.085945
TTTATAG35600.053.9697655
TATAAGG29350.053.8971447
TATAGGT41100.053.4753047
Produced by FastQC (version 0.11.5)