FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH109_MTS60mS3_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH109_MTS60mS3_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3038812
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG9500.057.8379485
CTTATAG20100.056.9362035
TTATAGG31800.056.6783146
CGTATAG14600.055.1330225
CTATAGG17650.054.726946
GTATAGG24450.053.677126
CCTATAC8550.053.6218765
CGTATAA15900.053.486725
ATAGGGC8750.052.7953348
TATAGGG29000.052.7367177
CGATAGT9450.052.5888066
TATAGGC20500.052.5807157
CGATAGG7100.051.7568446
TATAGGT37250.051.4853827
GATAGGT15250.051.405297
GGATAGT18000.051.329646
CTATAAC12000.051.0379946
TTATAGT36650.050.9922376
GCGATAG7500.050.3963745
TTATAGC22400.050.308886
Produced by FastQC (version 0.11.5)