FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH108_MTS60mS2_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH108_MTS60mS2_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13640125
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG167650.056.6767736
GTATAGG143350.056.17696
CTTATAG81500.056.082125
CGGATAG40150.055.4383625
CGTATAG68550.054.883255
TATAGGG163800.054.461557
CTATAGG82450.053.9927336
CGTATAA62600.053.7264185
TTATAGT191000.052.9362346
GGTATAG133050.052.7660875
CGATAGT43750.052.6364636
ATAGGGC50350.052.5480238
GCGATAG42650.052.270815
TATAGGT186650.052.256837
TATAGGC102600.051.8477947
CGATAGG37150.051.4366466
TATAGTG201850.051.390587
GTTATAG173750.050.779325
TTTATAG180350.050.2988055
GATAGGT90200.050.2067157
Produced by FastQC (version 0.11.5)