FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH107_MTS60mS1_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH107_MTS60mS1_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4768112
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG60850.058.494066
CGGATAG13000.057.0747345
CGTATAG26000.056.9401255
GTATAGG49150.056.895076
CTTATAG27850.056.802135
CTATAGG29950.055.7407656
TATAGGG59600.055.1387677
CGTATAA22350.054.9643365
TATAGGT66050.053.9402167
GGTATAG44850.053.688025
CGATAGT15000.053.6646086
ATAGGGC20450.053.0525328
CCTATAC9650.052.2259755
GTTATAG60200.051.858485
TTATAGT59800.051.5030446
TATAGGC34750.051.3633237
GATAGGT29500.051.2506647
TATAGTG65950.051.209467
TTATAAG43750.050.7180566
AGTATAG36300.050.714315
Produced by FastQC (version 0.11.5)