FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH103_MTS45mS3_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH103_MTS45mS3_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978412
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA8950.060.2171175
CGGATAG6250.058.7938045
TTATAGG24200.057.8451426
GTATAGG20150.057.835096
CTATAGG13650.057.6862226
TATAGGG22500.056.305187
CGTATAG10500.055.99415
CTTATAG11700.055.635165
CGATAGG6250.055.434166
TATAGGT27150.055.0402377
GCGATAG6200.054.752295
ATAGGGC7850.053.4975478
GCGATAA5000.053.1943935
TTATAGT22600.053.113876
ACGATAG4750.053.047045
GATAGGT11650.052.5695657
TATAGTG24850.052.2480137
TATAGGA20950.052.118617
GCGTATA11350.052.1090474
ATAGGCG5600.051.869538
Produced by FastQC (version 0.11.5)