FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH102_MTS45mS2_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH102_MTS45mS2_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11260977
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG33350.059.8170435
TTATAGG131050.059.3404776
CGTATAG56800.058.7206045
CTTATAG65100.058.27665
GTATAGG112150.057.981636
TATAGGG125200.057.2489747
CGTATAA55000.057.2061775
CTATAGG72150.056.6080936
GGTATAG96800.055.4258965
TATAGGT149050.055.015167
TTATAGT143550.054.4901926
TATAGGC83450.054.2268337
CGATAGT35300.053.538116
GCGATAG33000.053.3457685
GCGTATA66450.053.2476124
CCTATAC24600.053.208675
TATAGTG153500.053.0782627
CGATAGG30400.053.072726
ATAGGGC47050.052.813238
GTTATAG136700.052.6893425
Produced by FastQC (version 0.11.5)