FastQCFastQC Report
Tue 12 Feb 2019
HTVC5BGX9_n01_JH101_MTS45mS1_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTVC5BGX9_n01_JH101_MTS45mS1_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3980924
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG48550.058.8202636
CTATAGG25800.056.4286546
GCGATAG13600.056.3548435
CTTATAG25000.056.274565
CGGATAG12300.055.7669645
GTATAGG46000.055.385956
CGTATAA21400.055.1114965
CGATAGT12750.054.896656
CGTATAG23200.054.3050965
TATAGGG49550.054.102417
TATAGGT54450.053.989957
ATAGGGC14050.053.80338
GATAGGT25750.053.548897
GTTATAG49950.052.8977855
TATAGGC29350.052.823497
TTATAGT54200.052.8177726
CGATAGG11450.052.571336
GGTATAG45500.051.302735
TATAGTG61900.051.2800147
TATAGGA39350.051.2282187
Produced by FastQC (version 0.11.5)