FastQCFastQC Report
Mon 23 Jul 2018
HTV3TBGX5_n03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTV3TBGX5_n03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189919562
Sequences flagged as poor quality0
Sequence length99
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19041601.002613938210325No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG2691190.1417015694254813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5983950.069.119091
GGTATCA6202650.064.6154258
GTGGTAT6271400.063.627726
AGCAGTG6440300.063.1941382
GTATCAA6475100.061.1772659
AGTGGTA6624050.060.945645
TGGTATC6613600.059.460777
GCAGTGG7203250.056.8485873
CAGTGGT7153900.055.853274
ATCAACG6167500.035.40625410-11
ACGCAGA6595050.033.84604314-15
CAACGCA6537600.033.6030512-13
TCAACGC5996700.033.37695312-13
AGTACAT6733200.032.53933720-21
TACATGG6669050.032.25250222-23
AGAGTAC7007750.031.97026818-19
TATCAAC6248800.031.90285710-11
CGCAGAG6827100.031.20607216-17
GCAGAGT7235700.031.00909416-17
AACGCAG6700400.030.82203914-15
Produced by FastQC (version 0.11.2)