FastQCFastQC Report
Mon 23 Jul 2018
HTV3TBGX5_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTV3TBGX5_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189919562
Sequences flagged as poor quality0
Sequence length8
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCGTC5177201427.259969144200113No Hit
GGTTTACT4237439522.311759017220144No Hit
CTAAACGG2319270212.211855248486724No Hit
AACCGTAA186803549.835929381513631No Hit
GGGGGGGG29713231.5645165609638463No Hit
ACCCGTAA29397691.5479021587044308No Hit
TCGGCGGC28314671.4908769640064776No Hit
CTAACCGG15203520.8005241713857785No Hit
CACCGTAA11019680.5802288023389607No Hit
CTAATCGG10120320.5328740174748297No Hit
TCGGGGTC9977950.5253776859489598No Hit
ATCCGTAA9900300.5212891129140241No Hit
GGGTTACT9264450.4878091494334849No Hit
GGTTTACG9239840.4865133376834557No Hit
CTCCGTAA7368540.38798215004307984No Hit
TCGGCGTG5949080.3132420871947883No Hit
AACCGGAA5475460.28830416110584756No Hit
TCGGCGGG5162410.27182086698367597No Hit
GGTTTCCT4794370.2524421365293587No Hit
GGTTTTCT4475840.2356702991975097No Hit
CTACCCGG4419030.23267903282127408No Hit
AACCCGTC3754270.19767684594807564No Hit
ACCCCGTC3662300.19283426948931148No Hit
GGGGCGGG3589830.18901844350293942No Hit
CTAATACT3385900.17828073971653324No Hit
CTACACGG3365360.17719922921894693No Hit
TCCCGTAA3225850.1698534877623612No Hit
GGTGTACT3170920.16696121066243824No Hit
GGTTACGG3098400.1631427519825472No Hit
GGTTTCCG2996980.15780259644870073No Hit
CGAATACT2963030.15601499754933093No Hit
GCGGCGGC2922500.15388093618286672No Hit
ACGGCGTC2848520.14998560285222226No Hit
GGTTTCGG2833660.1492031663383891No Hit
TCGGCGAC2760520.14535206225886305No Hit
GGTTTAGT2722110.14332962709760252No Hit
GGTTAACT2704450.14239975974670793No Hit
AACCGTTA2698270.1420743588277652No Hit
TCCGCGTC2680100.14111763800297727No Hit
TCGCCGTC2669020.14053423311917707No Hit
CCCCGTAA2622990.1381105754656279No Hit
GGTATACT2473930.13026198954692197No Hit
TCGGGTAA2385150.1255873789346671No Hit
CTTAACGG2323610.1223470597515384No Hit
CGAAACGG2265680.11929682104047817No Hit
CTCCGTAT2207660.11624184348108385No Hit
TCGGCGTA2137260.11253501100639648No Hit
GCGGCGTC2122150.11173941102496855No Hit
AACCCTAA2089930.11004290332135454No Hit
CTAAACCG2085540.10981175283039037No Hit
GGTTTACA2076470.1093341822260521No Hit
GGGGTACT2045540.1077055980152271No Hit
GGATTACT2037100.10726119934922765No Hit
TCGGCCTC1985470.10454268002155565No Hit
CGTTTACT1939430.10211849582930273No Hit
CTATACGG1922630.10123391080693415No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)