FastQCFastQC Report
Mon 23 Jul 2018
HTV3TBGX5_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTV3TBGX5_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189919562
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAATG677150.018.4555841
CCGGTAG628650.018.0416431
CCACTAC1005650.017.747761
CCTTACG725450.017.5282331
TTGAACG416850.017.1437261
TGGGCGT407750.017.0622631
CGGACAC471100.017.0194111
TTGGCGC442200.016.96809210
AGGCTAG861550.016.9126118
CTGATAG639900.016.9030441
CCTAGCT1086600.016.9006041
AAAGGCG416400.016.9004549
CTTAGGA543150.016.8655931
ACGAGGA376050.016.7953171
CCCTCCT754750.016.776741
CGTCAGG797300.016.7740331
AGGGTGA444650.016.729131
AGAGCGA476500.016.6831341
CAGGAAT718650.016.6789998
CGATCGG529200.016.6228581
Produced by FastQC (version 0.11.2)