FastQCFastQC Report
Mon 6 Aug 2018
HTV3NBGX5_n03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTV3NBGX5_n03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490480369
Sequences flagged as poor quality0
Sequence length99
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46918050.9565734525860301No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG22756880.4639712705810658No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA20666180.4213457113917642No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTG8872560.1808953132638016No Hit
GCTAAATAAACAAGAAAGCAAAATCAACAACCGCAAAAGTCTAAGACATG5944830.12120423926691345No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCT5212630.10627601693065927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG16099800.070.458262
AAGCAGT16493950.068.989541
CAGTGGT17673750.063.9886054
AGTGGTA18018950.062.799065
GCAGTGG18479100.061.4660953
GTGGTAT18987250.059.59756
GGTATCA19318050.058.6256648
TGGTATC19653300.057.4979177
GTATCAA24661200.045.8021559
CATGGGG13456450.033.66333424-25
AGAGTAC18883750.033.26187518-19
GCAGAGT19122450.032.9146816-17
TACATGG19169600.032.4866822-23
CAACGCA20014450.031.49825912-13
ACGCAGA20025850.031.48962214-15
ATCAACG20302600.031.06898910-11
AGTACAT20770750.030.13976120-21
CGCAGAG19212550.029.72032216-17
CAGAGTA19333600.029.38154618-19
CCTCACT4628250.029.23191572-73
Produced by FastQC (version 0.11.2)