FastQCFastQC Report
Mon 6 Aug 2018
HTV3NBGX5_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTV3NBGX5_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490480369
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCATCA374384027.633007224393114No Hit
CCTGTGCG340188016.935812960130929No Hit
CAACGCCT332426816.777576249947733No Hit
GGACACGT318267376.48889109769855No Hit
AGTTCGGC313703116.395834162325058No Hit
CTAGACCT311530196.35153228731974No Hit
TGTGCGGG306450136.2479591308576925No Hit
TACTCTTC306246626.243809933196327No Hit
GAGATGAC296865846.052552941216695No Hit
TCCGGAAG273642255.579066305098135No Hit
ACTTCATA271894105.543424715536373No Hit
ATGAGAAA244105324.976862183040806No Hit
ACCATAAC236014484.8119047145799225No Hit
GGGGGGGG231913834.728299941398878No Hit
TGCCGTGG220212144.489723828274155No Hit
GTAATCTT218114274.446952085864216No Hit
GTGTATTA120678092.4604061166819093No Hit
CTAGCCCT9096380.1854585947760939No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)