FastQCFastQC Report
Mon 6 Aug 2018
HTV3NBGX5_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTV3NBGX5_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490480369
Sequences flagged as poor quality0
Sequence length27
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA1386950.017.8545078
GACTAAC1312100.017.8525661
CTGCGGA1105450.017.7553251
GGGACCT1124300.017.5819171
GGGAATG1134450.017.4746191
ACTGATG1209100.017.3393341
CTCGAAA1236600.017.3139481
TGACGGC1211300.017.2896291
GACCTGG1358200.017.2798751
GCGCGAT967500.017.228111
CGTGAGC1059150.017.1666471
GAATGAA1249750.017.1628721
ACGAGGA1183450.017.1487871
CGCTGGA1132300.017.1236841
TGACTAG1261000.017.118061
CGGACTG1109350.017.1000821
GATGAAA1320500.017.0643521
TGGGCGT1053600.017.009791
GACTGCG1198000.016.9747121
GGCTCGA1244750.016.9718631
Produced by FastQC (version 0.11.2)