FastQCFastQC Report
Sat 9 Feb 2019
HTTKYBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTTKYBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21509361
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1583950.7364003049648942No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG236170.10979870578210112No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG232970.10831098143733792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC119650.053.7588513
ACGTCTG119050.052.20735515
GCACACG127900.051.7964811
CACGTCT122350.051.7716214
CGTCTGA120400.051.44877216
CACACGT129250.051.0930112
GATCGGA149700.049.7739221
ATCGGAA152100.049.6152232
CGGAAGA153450.047.846114
TCGGAAG155300.047.6595543
AGAGCAC170250.041.2745748
AGCACAC176400.039.3994110
GAACTCC153000.038.63730621
CTGAACT165100.036.7986519
TGAACTC174800.034.6974420
AACTCCA188800.031.14599222
CGGGGGG54500.030.8552531
CTCCAGT171700.029.88626924
CCAGTCA142750.028.86113526
TCTGAAC220400.028.23171818
Produced by FastQC (version 0.11.5)