FastQCFastQC Report
Sat 9 Feb 2019
HTTKYBGX9_n01_mb175.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTTKYBGX9_n01_mb175.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27542857
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT27900.013.4249821
CGCGTTT12650.012.4524881
CGTATTA11950.012.30069353
CCCGTTT53500.010.4688831
CTCGTTT71900.010.4675181
CTCGAAT77400.010.35687351
CTCGTAT45950.010.2844811
CTCCGTT70900.010.0226821
CGCGGTT14750.09.9676181
CGGGAAT36250.09.9463411
ACGATAC26700.09.56905960
CGCATAT14000.09.5014461
CTCCGGT76650.09.4991631
CCCGAAT45000.09.4903331
CCCGTCT77650.09.4219111
CCCCGCT79900.09.3318331
CTCCGAT73100.09.1463991
TTAACCG18000.09.1383727
CGCCTAT10550.08.958711
ACGTATA19950.08.94715418
Produced by FastQC (version 0.11.5)