FastQCFastQC Report
Sat 9 Feb 2019
HTTKYBGX9_n01_mb174.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTTKYBGX9_n01_mb174.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26699027
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAT12550.011.1566031
CCCGTAT26600.010.6590641
ACGTATA18350.010.4901618
CCCGATT26150.010.0393431
GACGTAT18800.09.68069817
CCCGTCT70950.09.571171
CTCCGCT94650.09.3935291
CTCCGTT63700.09.2867331
CTCGAAT74500.09.1620791
CTCCGGT71650.09.0379771
CGGGAAT34500.09.0299771
CGTATTA10900.08.9904323
CCCGTTT53950.08.9537171
CTCCTAT62600.08.9466681
CCGGTAT23850.08.8059981
CTCGTTT70850.08.7941891
TACGCGA4804.4921006E-58.7495359
CCGGGAT53800.08.5883031
CGCTATT14750.08.543311
CCCGAAT45850.08.4742131
Produced by FastQC (version 0.11.5)