FastQCFastQC Report
Sat 9 Feb 2019
HTTKYBGX9_n01_mb173.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTTKYBGX9_n01_mb173.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28972506
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA13000.013.7301333
CCCGTAT29000.012.6740361
CCCGAAT51400.010.351521
CCCGTTT56750.010.2391971
CTCCGAT76550.09.5113421
CCGTATT36650.09.262922
CTCGTTT73100.09.2419491
GACGTAT20000.09.09986117
TATGCCG20500.09.04812848
CTCCGCT105000.09.0011521
CCCGATT28300.08.9057331
CCCCGCT84100.08.86558151
CCGGTAT27300.08.8472871
GTCCGGT42900.08.8123171
CCCGTCT76650.08.7225781
CGCATAT16300.08.5900561
CCCCGAT51850.08.5739041
CCCGCAT51650.08.4715591
GTCCGTT37750.08.4381671
CTCGTAT47800.08.421581
Produced by FastQC (version 0.11.5)