FastQCFastQC Report
Sat 9 Feb 2019
HTTKYBGX9_n01_mb172.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTTKYBGX9_n01_mb172.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26818709
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28550.026.35533148
TCGTATG34700.021.88612245
CGTATGC38350.019.98549346
AATCTCG58750.015.66716641
ACACGTC79950.015.01466913
ACGTCTG81200.014.78361615
CTCGTAT55200.014.58236944
CGTCTGA85950.014.13003316
GTATGCC56750.013.93730147
AATCGCG11000.013.04468941
CCCGTAT27650.012.7862531
CACGTCT97050.012.51338814
CGTATTA9750.012.2044353
CACACGT102450.012.02471812
ATCGCGT9300.011.28963942
ATGCCGT69250.011.11833749
TCTCGTA74350.011.01475943
CGCGTAT8200.010.24322544
GCACACG123450.010.17765111
GATCGGA117050.09.9584081
Produced by FastQC (version 0.11.5)