FastQCFastQC Report
Sat 9 Feb 2019
HTTKYBGX9_n01_mb171.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTTKYBGX9_n01_mb171.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28682079
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT27750.011.7310121
CGTATTA12350.010.7686423
CTCGTTT72350.09.4343411
CCCGTTT58850.09.3977991
CGGGAAT37600.09.1233381
CCCGTCT77700.08.9649441
CCCGATT27300.08.847121
CCGTATT35550.08.7619052
GTCCGTT42050.08.7405511
CTCGTAT46950.08.6484421
GTCCGGT37100.08.5858441
CGATCTA12450.08.4332754
GTCGGTT50500.08.3870551
CTCGAAT83900.08.26073551
CGCTAAT15750.08.00087451
CTCCGAT73000.07.9118241
CGCATAT15500.07.9040891
CGGGTAT23700.07.827861
GTCCGCT57750.07.8190361
CGCTAGT9901.029548E-97.7786281
Produced by FastQC (version 0.11.5)