FastQCFastQC Report
Sat 9 Feb 2019
HTTKYBGX9_n01_mb170.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTTKYBGX9_n01_mb170.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29771771
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT28600.011.7493141
CCCGAAT47200.010.0114991
CTCCGCT97450.09.6622381
CTCGTAT49000.09.4294231
CGCCTAT12350.09.3530721
CGTATTA11300.09.2915023
CCCGTTT60700.09.1688681
GTCGCAT26050.09.1371021
CCCGCAT48600.08.7868031
CTCGAAT85250.08.7867181
CGGGTAT25500.08.7851081
CCCGTCT80950.08.7778171
CGCGTTT12550.08.64621
CCGGTAT27300.08.590521
CTCCGAT69700.08.5875771
CCCCGCT82000.08.4519831
CTCAGAT149150.08.4486351
CCCGTAC31150.08.4277291
CTCCTAT72500.08.400761
ACGATAC29200.08.39018360
Produced by FastQC (version 0.11.5)